Screening and analysis of key active constituents in Guanxinshutong capsule using mass spectrum and integrative network pharmacology / 中国天然药物

Guanxinshutong capsule (GXSTC) is an effective and safe traditional Chinese medicine used in the treatment of cardiovascular diseases (CVDs) for many years. However, the targets of this herbal formula and the underlying molecular mechanisms of action involved in the treatment of CVDs are still unclear. In the present study, we used a systems pharmacology approach to identify the active ingredients of GXSTC and their corresponding targets in the calcium signaling pathway with respect to the treatment of CVDs. This method integrated chromatographic techniques, prediction of absorption, distribution, metabolism, and excretion, analysis using Kyoto Encyclopedia of Genes and Genomes, network construction, and pharmacological experiments. 12 active compounds and 33 targets were found to have a role in the treatment of CVDs, and four main active ingredients, including protocatechuic acid, cryptotanshinone, eugenol, and borneol were selected to verify the effect of (GXSTC) on calcium signaling system in cardiomyocyte injury induced by hypoxia and reoxygenation. The results from the present study revealed the active components and targets of GXSTC in the treatment of CVDs, providing a new perspective to enhance the understanding of the role of the calcium signaling pathway in the therapeutic effect of GXSTC.

Therapeutic Observation of Collateral-pricking Bloodletting Therapy plusXiao Feng San for Chronic Urticaria Due to Blood Deficiency and Wind Dryness / 上海针灸杂志

Objective To observe the clinical efficacy of collateral-pricking bloodletting therapy plusXiao Feng San in treating chronic urticaria due to blood deficiency and wind dryness.Method A total of 150 patients with chronic urticaria due to blood deficiency and wind dryness were randomized into a treatment group and a control group, 75 cases each. The treatment group was intervened by collateral-pricking bloodletting plus oral administration ofXiao Feng San, while the control group was intervened by oral administration ofXiao Feng San alone. Before and after 4-week treatment, the serum indexes [immunoglobulin E (IgE), eosinophil count, C3, C4, interleukin (IL)-2, IL-4] of the two groups were observed, and the clinical efficacies were also compared.Result The total effective rate and markedly effective rate were respectively 100.0％ and 86.7％ in the treatment group versus 80.0％ and 52.0％ in the control group, and the between-group differences were statistically significant (P<0.05,P<0.01). After the treatment, the serum indexes were significantly changed in the treatment group (P<0.05). The levels of IgE, eosinophil count, IL-2 and IL-4 were significantly changed after the treatment in the control group (P<0.05). There were significant differences in comparing the serum indexes between the two groups after the intervention (P<0.05).Conclusion Collateral-pricking bloodletting therapy plusXiao Feng San is an effective method in treating chronic urticarial due to blood deficiency and wind dryness, and it can improve the serum indexes.

Screening and analysis of key active constituents in Guanxinshutong capsule using mass spectrum and integrative network pharmacology / 中国天然药物

Guanxinshutong capsule (GXSTC) is an effective and safe traditional Chinese medicine used in the treatment of cardiovascular diseases (CVDs) for many years. However, the targets of this herbal formula and the underlying molecular mechanisms of action involved in the treatment of CVDs are still unclear. In the present study, we used a systems pharmacology approach to identify the active ingredients of GXSTC and their corresponding targets in the calcium signaling pathway with respect to the treatment of CVDs. This method integrated chromatographic techniques, prediction of absorption, distribution, metabolism, and excretion, analysis using Kyoto Encyclopedia of Genes and Genomes, network construction, and pharmacological experiments. 12 active compounds and 33 targets were found to have a role in the treatment of CVDs, and four main active ingredients, including protocatechuic acid, cryptotanshinone, eugenol, and borneol were selected to verify the effect of (GXSTC) on calcium signaling system in cardiomyocyte injury induced by hypoxia and reoxygenation. The results from the present study revealed the active components and targets of GXSTC in the treatment of CVDs, providing a new perspective to enhance the understanding of the role of the calcium signaling pathway in the therapeutic effect of GXSTC.

Evaluation on surface cleanliness of personal digital assistant as well as cleaning and disinfection efficacy / 中国感染控制杂志

Objective To investigate the cleanliness status of personal digital assistant(PDA)surfaces, and observe the effect of disposable antiseptic wipes on the cleaning and disinfection of PDA surface.Methods From January to March 2016, 83 daily used handheld PDA in 50 clinical wards of a hospital were as control group, 83 PDA disinfected by disposable antiseptic wipes(CaviWipes)and used in the same environment of the ward at the same time were selected as the intervention group, cleanliness status of PDA surfaces of two groups were detected by adenosine triphosphate(ATP)bioluminescence assay.Results Taken a cut-off value≤100 RLU/100 cm2 as qualified standard of cleanliness, none of PDA in control group was qualified with ATP bioluminescence reading ranged from 189 to 3 379 RLU;after disinfection with CaviWipes, the qualified rate of intervention group was 90.4％(n=75), ATP detection value of PDA surface in all departments were significantly lower than control group(median:[18-28] RLU vs[290-339]RLU, all P＜0.05).Conclusion Microbial contamination on PDA used in hospital wards is common, antiseptic wipes containing quaternary ammonium salt can be used for cleaning and disinfecting surface of PDA.